CATEGORY NUMBER : 551/2015,224/2017,409/2017,457/2017,458/2017, 554/2017,635/2017,636/2017, 637/2017,638/2017, 639/2017, 7/2018
NAME OF POST : JUNIOR LABORATORY ASSISTANT/ JUNIOR LAB ASSISTANT / LABORATORY TECHNICIAN GRADE II
DEPARTMENT : HEALTH SERVICES AND MEDICAL EDUCATION SERVICE
EXAMINATION DATE : 07-07-2018 SATURDAY
EXAMINATION TIME : 1:30 Pm to 3.15 Pm
MAXIMUM MARK : 100
EXAMINATION DURATION : 1.15 HRS
MEDIUM OF QUESTIONS : ENGLISH
SYLLABUS : An Objective Type Test (OMR Valuation) based on the qualification prescribed for the post.
Part I : MLT
Part II : Physics
Part III : Chemistry
Part IV : Biology
Part V : General Knowledge, Current Affairs & Renaissance in Kerala
Candidates can download the Admission Tickets through their One Time Registration Profile : 23-06-2018
PART - I
Blood and Phlebotomy Methods of blood collection - Capillary Puncture Arterial puncture and venous puncture Sites of blood collection: Capillary and Venous. Identification of blood cells: Leishman's stain Blood cell types: RBC, WBC, Platelets (Identifying characters and Normal Range )
Hematology Heamocytometry Counting chamber : improved Neubauer Total cell counts: RBC,WBC,Platelet,Absolute Eosinophil count. (Diluting fluids, Normal value, Clinical Significance), Reticulocount : Clinical significance Hemoglobin estimation : Enumerate methods ,WHO recognized method : cyanmethhaemoglobin method,significance. PCV – Methods, Wintrobe 's method, Normal value clinical significance. ESR- Method (westergren's method), Normal value,Clinical Significance. Test for coagulation: Bleeding time methods & Normal value of Duke's method. Clotting time methods & Normal value of Capillary tube method Automation Heamotology analyser, Haemogram, CBC
Blood Banking and Immuno haemotology Blood group antigens and antibodies. Blood group system. (ABO and Rh system in detail) Inheritance of blood group. Hemolytic Disease of New Born (HDN ) Blood grouping techniques. Cell grouping and Serum grouping. Slide and tube method Transfusion phlebotomy Donor screening , Donor selection criteria. Blood bank-Anticoagulants. Blood components. Compatability testing and issue of blood. Cross matching-Minor and Major. Mention 3 phases. Transfusion reaction:Name only.
Clinical Pathology Analysis of Urine, Sputum, Stool, Semen, CSF (physical,Chemical, Microscopic examination, Importance)
Biochemistry Blood Glucose estimation Types of samples: FBS, PPBS,RBS Methods of estimation.Normal value, Clinical ignificance, Relevance of HbAlc Renal function test Enumerate the common test included. Common method used for the estimation of Blood urea, Serum creatinine (normal values). Clearance test (Urea & Creatinine) Liver function test Common tests included. Method used for estimation of Bilirubin (Malloy and Evelyn method) Lipid profile Tests included in lipid profile Common method for the estimation of cholesterol. Other parameters of Diagnostic importance. Serum electrolytes: Sodium, Potassium. Normal value and Clinical significance. Name cardiac Markers. Name Tumor Markers.
Microbiology Classification of bacteria based on Morphology. Sterilization methods. Hot Air Oven, Autoclave (Use ) Disinfectants & Antiseptics (Application) Culture Media: Classification of culture media with example. Culture Methods: Streak , Stroke, Stab, Lawn culture, Anaerobic technique (gaspak) Identification of bacteria : Different methods . Detection of motility : Names of different methods (Hanging Drop Method ) Staining : (Simple stain, gram stain, AFB stain) Diagnostic significance. Biochemical tests : Coagulase, Catalase, & IMViC Immunology Types of immunity, types of antibody (IgG, IgA, IgM, IgD, IgE)
Parasitology Laboratory diagnosis of Malaria (Disease, Mode of transmission, host, causative agent. Types of Malaria, Thick and thin smear. Other stains used: JSB, Other methods : card method ,QBC. Laboratory Diagnosis of Filariasis . (Disease, mode of transmission,host and nocturnal habit. Lab diagnosis : Wet smear examination , thick smear examination, concentration techniques. Histotechnology And Cytology Histotechnology :Tissue processing (steps of tissue processing), Microtomes, staining: (H and E stain) Diagnostic cytology : Types of specimen , Processing , Fixation (fixatives used), Staining, advantages and application of diagnostic cytology
PHYSICS Units and measurement : Units for measurement, CGS Unit , SI unit, Accuracy and errors in measurement, Significant figures Motion in one, two and three dimensions: Distance and displacement, Speed and velocity, Acceleration, Equations of motion, Projectile motion, Uniform circular motion Laws of Motion : Newtons laws of motion, Force, Inertia, Linear momentum and its conservation, Friction Work, Energy and Power : Work, Energy, Different forms of Energy, Kinetic Energy, Potential energy, Conservation of energy, Power Rotational motion : Centre of Mass, Angular momentum and its conservation, Torque, Principle of moments, Moment of Inertia, Rotational kinetic Energy Gravitation : Mass and weight, Acceleration due to gravity and its variation with height and depth Mechanics of solids and liquids : Elastic properties of solids, Stress, Strain, Hooke's law, Modulus of Elasticity, Fluid pressure, Pascal's law, Buoyancy, Surface tension, Viscosity, Capillary rise, Bernoulli's theorem and its applications Heat: Measurement of temperature,Thermal expansion, Specific heat capacity, Latent Heat, Transfer of heat, Newton's law of cooling. Waves: Longitudinal and transverse waves, Speed of wave motion, Reflection of waves, Echo. Electrostatics: Electric charges and their properties, Coulomb's law, Electric field, Electric potential, Capacitance, Capacitors in series and parallel, capacity of a parallel plate capacitor Current electricity : Electric current, Ohm's law, Resistance and resistivity, Resistance in series and parallel, Carbon resistors, Kirchoff's Magnetism : Earth'magnetism, para, dia and ferromagnetic substances, Electromagnets and permanent magnets. Electromagnetic induction: Faraday's law, Lenz's law, Eddy current, Self Induction, Mutual induction Alternating current: AC generator, LCR circuit, Reactance, Impedence, Resonance, Transformer Electromagnetic waves : Electromagnetic waves and their properties, Electro magnetic spectrum and their uses Optics: Reflection, Refraction, Total internal reflection, Refraction through prism, Refraction through lens, Lenses in contact, Magnification, Power of lens, Dispersion, Scattering, Interference, Diffraction, Polarisation, Microscope, Telescope Dual nature of matter and radiations: Photoelectric effect, Matter wave Semiconducters: Conducters, insulators and semiconductors, Intrinsic semiconductors, Extrinsic semiconductors, Diode, Transistor
Periodic classification of elements – Earlier classifications - Modern Periodic law and Periodic Table - various groups in Modern Periodic Table Classification of elements in blocks- Periodic properties and their variation along group and period with reason Allotrops of Carbon - Diamond and Graphite their structure and properties Compounds of Nitrogen -Ammonia and Nitric acid, compounds of sulphur -sulphuric acid Lanthanoids and Actinoids-Interhalogen compounds Types of water-Hardness of water-causes of hardness and its removal Different atom models by J J Thomson, Rutherford, Neils BohrQuantum numbers and orbital representation-orbit and orbitalElectronic configuration and Auf-bau principle Colloids and emulsions-type of colloids with examples-colloids around us Ores of different metals -methods of extraction of metals VSEPR Theory and shapes of molecules Seven crystal systems with examples Different types of electrochemical cells Different methods used for purifying organic compounds – distillation, crystallisation, sublimation Tests for alchohol, aldehydes, ketones, phenol Molarity, molarity calculations Mole concept calculations Chemicals in food, medicines and as cleansing agents Green house effect-acid rain Different polymers with examples Hydrocarbons-general formula -structural representation
PART – IV
1. Diversity in Living World
Biodiversity, Taxonomy, Taxonomic hierarchy, Binomial nomenclature, Tools for study of Taxonomy - Museums, Zoos, Herbaria, Botanical gardens Biological classification -Five kingdoms, virus, viroids and lichen Plant Kingdom - Brief classification of plants with examples
2. Structural Organisation in plants
Plant morphology - root, stem and leaf structure & modification, Flower morphology, floral diagram and floral formula of Fabaceae, Solanaceae and Liliaceae Plant anatomy - Internal structure of plant, tissues, tissue systems, Primary structure of stem, root & leaf. Secondary growth in stem and root
3. Cell and cell division
Structure of plant and animal cell, various cell organelles and their function. Biomolecules-structure and function of proteins, carbohydrates, lipid, nucleic acids; Enzymes-types, properties and mode of action . Cell division-cell cycle,mitosis &meosis
4. Plant Physiology
Transport in plants -cell to cell transport, plant water relations, transport of water and minerals, transpiration and its mechanism, guttation and translocation of minerals. Plant nutrition-classification of minerals in plants, their role, deficiency symptoms, nitrogen cycle, biological nitrogen fixation. Photosynthesis-photosynthetic pigments, photochemical phase-cyclic and non cyclic photophosphorylation, biochemical phase-photorespiration, C3 and C 4 cycles. Factors affecting photosynthesis. Respiration-aerobic & anaerobic respiration, steps in cell respiration–Glycolysis,TCA Cycle, Terminal oxidation, energy relation and respiratory quotient. Plant growth and development: Phases of plant growth; Differentiation, dedifferentiation and redifferentiation; Growth regulators-auxin, gibberellin, cytokinin, ethylene, ABA, Seed dormancy; Vernalisation and Photoperiodism. Reproduction in flowering plants: Development of male and female gametophytes; Pollination - types,agencies and examples.Outbreedings devices; Double fertilisation; Post fertilisation events-Development of endosperm and embryo and seed ,Special modes - apomixis, parthenocarpy, polyembryony.
5 Ecology and environment
Organism and Environment:Environmental factors, Ecological levels-species,population & community, ecological adaptations. Population attributes-growth birth rate and death rate and age distribution. Population interactions. Ecosystem: Structure and function, Productivity,energy flow, food chain, food web and ecological pyramids. Biogeochemical cycles, Ecological succession. Environmental issues : Air, water, soil and noise pollution. Solid waste management,radioactive waste management. Green house and global warming ,ozone depletion. Zoology
1 Animal Physiology
Animal nutrition and its type, food components, malnutrition, disorder related to nutrition. Human digestive system and process of digestion Respiration :Respiratory organs,Respiration in man – mechanism and its regulation, gas exchange, respiratory volume and respiratory disorders Circulation:Blood and its composition. Human heart -structure and working ,double circulation, ECG, circulatory disorders Excretion:Structure and function of human kidney, osmoregulation, regulation of urine formation. Other excretory organs Locomotion and Movements : Human skeleton , types of joint, Types of muscle and structure, Mechanim of muscle contraction and disorders Nervous co- ordination : Human Nervous system -structure and function of brain and spinal cord ,nerve impulse transmission .Sensory receptors - structure and function of eye, ear and tongue. Endocrine system : endocrine glands, hormones and their functions,hormonal imbalance and disorders. Reproduction and development:Human male and female reproductive system, menstrual cycle, gametogenesis fertilisation and embryogeny. Placenta and its function.
2 Genetics and Evolution
Heredity and variation : Mendelian inheritance. Non -Mendelian inheritance-incomplete dominance, co-dominance, multiple alleles and polygenic inheritance, Sex determination. Mendelian disorders and chromosomal disorders. Molecular basis of inheritance: Structure of DNA and RNA, Search for DNA as genetic material, Nucleosomes.Functions of DNA - replication, transcription and translation .Central dogma, genetic code. Gene expression and regulation -Lac operon. Evolution : Origin of life ,Theories of evolution, evidence of evolution , Natural selection and example, types of selection, Hardy-Weinberg's principle and human evolution
3 Animal Kingdom
Salient features and classification of animals, non -chordates and chordates up to classes level. Morphology and internal structure of Earthworm, cockroach and frog.
4.Biology and Human welfare
Birth control measures, Assisted reproductive technologies, STD Common problems of adolescence, problem associated with drugs, smoking and alcoholism
Animal and plant breeding, Tissue culture, Biotechnology principles and its applications in agriculture and medicine. Biopatent, Biopiracy, biofertilizers, sewage treatment, biogas and biomedical technologies.
General Knowledge,CURRENT AFFAIRS&RENAISSANCE IN KERALA
Facts about India Geography of India – Physical features – Climate – Soils – Rivers – Famous sites – etc. Demography – Economic and social development – Poverty alleviation – Economy and planning – etc. History of India – Period from 1857 to 1947 – National movement Five Year Plans
Facts about Kerala
Geographical Facts – Physical features – Climate – Soils – Rivers – Famous sites – Economic and Social deve – Historical importance - etc.
Renaissance in Kerala
Important Events/Movements/Leaders Brahmananda Swami Sivayogi, Chattampi Swami, Sree Narayana Guru, Vagbhatananda, Thycaud Ayya, Ayya Vaikundar, Poikayil Yohannan (Kumara Guru), Ayyankali, Pandit Karuppan, Mannathu Padmanabhan,V. T. Bhattathirippad, Dr. Palpu, Kumaranasan, Vakkom Moulavi, Blessed Kuriakose Elias Chavara, Etc.
Important world, national and regional events related to the political and scientific fields, sports, cinema and literature etc.
NOTE: - It may be noted that apart from the topics detailed above, questions from other topics prescribed for the educational qualification of the post may also appear in the question paper. There is no undertaking that all the topics above may be covered in the question paper